Photo:

David Christensen

It's almost all over. Just sent off some nominations for student prizes. Thanks for loads of great questions! It was tough to narrow down who to nominate.

Favourite Thing: I really enjoy writing or talking about the work I do. Within the lab, my favourite thing is when I get some nice-looking photos or when I try something new and it works!

My CV

Education:

University of Oxford 2006-2010, University of Southampton 2010-2014

Qualifications:

Biochemistry degree (MBiochem)

Work History:

Washed dishes during university holidays at Athelhampton House restaurant in Dorset 2007-2010!

Current Job:

Final year PhD Student

Employer:

University of Southampton

Me and my work

I’m looking at how stem cells control how much sugar they eat.

I work with human embryonic stem cells, but unlike a lot of scientists who might want to find ways to produce hearts, livers, kidneys or brains from their stem cells, I’m trying to find out how we can make a stem cell stay as a stem cell. A lot of the time stem cells decide (without us telling them what to do) to become a different cell type, such as a muscle cell or a heart cell. Sometimes different stem cells that are next to each other might become different types of cells. This can be a problem if you have stem cells that you want to make into a liver, but some of them are already becoming heart and kidney, because a mix of different types of cells would be useless for treating your patient with liver disease. Because of this, some scientists, including me, are trying to find what makes stem cells happy to stay as stem cells.

One thing we know about stem cells is that they consume a lot of glucose sugar. When the stem cells become other types of cells they start to consume a lot less sugar. We are trying to find out how this is controlled and how we can affect how much sugar the stem cells consume. One thing that we know affects how much sugar the stem cells need is how much oxygen is available to the stem cells. All cells in our bodies need oxygen to live and get oxygen from the blood, but some cells need and get more oxygen than others. It seems that stem cells need less oxygen than other cells and giving them more oxygen than they need changes the way they behave and makes them less happy to stay as stem cells. At the same time, giving them more oxygen than they need makes stem cells consume less sugar. I’m interested in how and why having more oxygen makes stem cells consume less sugar and makes them less happy to be stem cells.

My Typical Day

The stem cells I use need to be carefully looked after every day so I spend a lot of time looking at them.

One great thing about my work in a lab is that I can start work whenever I want in the morning! Generally I start work at 9 and go home at 5, but some days I get in later and some days I leave early – on the best days I get to work late and leave early!

To keep the stem cells happy, I need to feed them every day and move them onto new plates every few days so that they don’t grow on top of each other. This means that I have to look at them every day to see whether I think they just need to be fed or whether there are too many of them on that plate and I need to take some of them to go onto a new plate where they will have more space. Because the stem cells are so needy I have to go into the lab to do some work on weekends quite often, which is really annoying!

Although feeding the stem cells is something I do every day, it isn’t actually the work I do to try to learn new things about the stem cells. To learn more about the how stem cells consume sugar and how they are affected by the amount of oxygen available, I have to do various experiments to compare stem cells that I have grown separately and differently. For example, I always have stem cells that are being grown with a lot of oxygen and also stem cells that have very little oxygen. These stem cells are grown in a liquid with sugar and other nutrients in it that the stem cells need to grow. If I know how much glucose sugar there is in this liquid before I put the stem cells into it and then I can measure how much sugar is left in it a bit later, then I can work out how much sugar the stem cells have eaten from that liquid. As I do this experiment with stem cells that are grown in lots of oxygen and stem cells that are grown with very little oxygen, I can see whether the cells grown in less oxygen eat more or less sugar. Recently I found out that stem cells grown in less oxygen do eat more glucose sugar.

What I'd do with the money

I’d like to give the money to a charity to organise a stem cell themed interactive, fun, session for members of the public to come in to learn more about stem cell science.

I’m not sure exactly what I’d want to do with the money, but I have been reading about the fun events that EuroStemCell do at places like the Science Museum in London. They’ve run interactive events where members of the public can learn more about what stem cells are and what research is being done on stem cells. I think it would be really cool and fun to do something like that.

Another thing I could do with the money would be to organise a day when some local schools where I work could come in to look around the labs here. I’m going to be getting involved soon with something called LifeLab in Southampton. They give local school kids the opportunity to do some fun lab experiments, such as using ultrasound to look at their own arteries, as well as just arranging for us scientists to go to talk to the kids about the work we do in the lab.

My Interview

How would you describe yourself in 3 words?

Tall. Enthusiastic. Lazy.

Who is your favourite singer or band?

Too many favourites, but if I could only pick one band, then it would be Radiohead.

What's your favourite food?

Indian.

What is the most fun thing you've done?

Zorbing – the thing where you roll down a hill in a big ball with some water inside it too.

What did you want to be after you left school?

I had no idea, so I did a science degree just because I did fairly well in science classes at school.

Were you ever in trouble in at school?

Sometimes, but not too much.

What was your favourite subject at school?

Biology.

What's the best thing you've done as a scientist?

It’s really exciting when you see that work you have done has been published in a journal that will be read by other scientists all over the world and so far I’ve had that twice.

What or who inspired you to become a scientist?

I don’t remember any individual person or thing that happened to inspire me. I think it just happened without me noticing!

If you weren't a scientist, what would you be?

In my dreams I’d be in a band or at least write about music for a magazine, but probably I’d be even more boring than I am and be working in a bank or something!

If you had 3 wishes for yourself what would they be? - be honest!

I wish that at some point soon I find out what I want to do as a job when I grow up (yes I know you all probably think I’m grownup already, but I don’t). I wish I had just a little bit more money and I wish I had more time to meet up with all my friends around the country.

Tell us a joke.

The pollen count; now that’s a difficult job. Especially if you’ve got hay fever. (From comedian Milton Jones)

Other stuff

Work photos:

Here’s a picture of a plate of my cells inside a hood:myimage1

And this is the rest of the room where that hood is. This is one of the labs where I do most of my work:

myimage2

I baked these for our lab meeting – we take it turns each week to bake and bring in cake or biscuits myimage3

These are some human embryonic stem cells that I’ve grown in the lab. The blue parts are the nuclei of the cells and the green parts are some proteins found on the edge of the cells.

myimage4